Catheptic and Dipeptidase Activities of Ascites Tumour Cells

Purine-phosphoribosyltransferase activities in rat and mouse tissues and in Ehrlich ascites-tumour cells.

1. The activities of adenine phosphoribosyltransferase and hypoxanthine phosphoribosyltransferase in extracts of rat and mouse liver, brain, spleen, heart and kidney, of rat bone marrow and of Ehrlich ascites-tumour cells have been measured. The specific activity of adenine phosphoribosyltransferase in tumour cells (3mmu-moles of nucleotide formed/min./mg. of protein) was between 15 and 60 time...

Transport of calcium ions by Ehrlich ascites-tumour cells.

Ehrlich ascites-tumour cells accumulate Ca2+ when incubated aerobically with succinate, phosphate and rotenone, as revealed by isotopic and atomic-absorption measurements. Ca2+ does not stimulate oxygen consumption by carefully prepared Ehrlich cells, but des so when the cells are placed in a hypo-osmotic medium. Neither glutamate nor malate support Ca2+ uptake in 'intact' Ehrlich cells, nor do...

Effect of streptococcal haemolysins on Ehrlich ascites tumour cells.

Deoxyribonucleic acid nucleotidyltransferase from Landschütz ascites-tumour cells.

1. A purification procedure for DNA nucleotidyltransferase from Landschütz ascites-tumour cells is described. The enzyme can be separated from endogenous nucleic acid and from triphosphatase and deoxyribonuclease activities measurable at pH7.5. 2. The basic properties of the nucleotidyltransferase reaction are as follows. The enzyme has optimum activity at pH7.2-7.4. It displays an absolute req...

On the phagocytizing activities of Ehrlich ascites tumor cells.

Ehrlich ascites tumor cells actively phagocytize the pigments derived from benzidine yellow. The pigments were synthesized from acetoacet-arylide, and 5 derivatives with similar chemical structure were prepared by conjugation of various substitutes. Polyoxyethylene lauryl ether (a), polyoxyethylene nonylphenol ether (fe), conjugated material of naphthaliosulfonic acid and formaldehyde (c), poly...